On February 16, 2019, the first jaguar cub ever born from AI arrived at the Associação Mata Ciliar in Brazil, the Cincinnati Zoo & Botanical Garden’s Center for Conservation and Research of Endangered Wildlife (CREW) reported at the time. The birth was the result of an AI procedure performed by scientists from CREW and represented a major scientific advancement for the conservation of this species. The mother, a wild-born female named Bianca, was one of five jaguars inseminated in 2019.
In 2019, the first jaguar cub born through artificial insemination was tragically eaten by its mother
Share icon Image credits: Daley van de Sande Lindsey Vansandt, CREW’s theriogenologist and the lead scientist on the project, conducted the AI procedures with the assistance of CREW’s Director of Animal Research Dr. Bill Swanson. She said: “By using a systematic research strategy, we were able to improve our understanding of the jaguar’s unique reproductive biology and make species-specific modifications to our standard AI approach.” Lindsey continued: “The jaguar is the last of the seven big cat species to be produced by AI. “The birth of this cub is an important milestone and invigorates the possibility of using assisted reproduction as a management tool to conserve this iconic cat.” Share icon Image credits: zooborns Following AI with freshly collected semen from a resident wild-born male, Bianca conceived and, after a 104-day gestation period, gave birth to a single viable jaguar cub, CREW explained. Upon the successful birth, remote video monitoring showed excellent maternal care and nursing. However, the cub eventually disappeared from the maternity den two days after birth and was presumably consumed by mom Bianca. At the time, Lindsey explained: “From a scientific perspective, we’re celebrating the fact that the cub was born healthy and that the AI was a success.
Since then, a new attempt at inseminating the same female jaguar has been made
Share icon Image credits: Freepik “It’s disappointing that the cub did not survive longer, but it’s not uncommon for carnivores, especially first-time mothers, to behave this way with their offspring.” In 2021, Brazilian and American scientists attempted to repeat the same procedure with Bianca, along with another female jaguar at the same facility, named Tabatinga. At that point, Bianca was living in a protected area in Sao Paulo state, Brazil. Share icon Image credits: zooborns The 110-pound feline, who was eight years old at the time, was once again used to advance the cause of preserving her species, but results have remained unclear since the last reports of the procedures. Bored Panda has contacted the Cincinnati Zoo & Botanical Garden and Lindsey Vansandt for comment. “The population sort of becomes smaller and smaller, and then you get inbreeding which has lots of bad consequences,” Lindsey told The Associated Press (AP) moments after performing the procedure on an unconscious Bianca atop a surgery table, in 2021. She further stated: “If we can take sperm from one male and inseminate a female from another location, we can keep their gene flow moving and keep the population more healthy.”
The mother, a wild-born female named Bianca, was one of five jaguars inseminated in 2019
— Cincinnati Zoo (@CincinnatiZoo) March 14, 2019 Wildlife experts from the Cincinnati Zoo, the Federal University of Mato Grosso, and the environmental organization Mata Ciliar have developed their insemination program for the Western Hemisphere’s largest feline for years, the AP reported. Some jaguars badly injured by blazes in the Pantanal (a natural region encompassing the world’s largest tropical wetland area) in 2020 required transport to specialized facilities for care. Others either died or were displaced, according to the AP. Cristina Adania, a veterinarian and coordinator of Mata Ciliar, told the AP: “Look what happened in the Pantanal, the Cerrado. “They are being killed before we even get to treat them, so something has to be done.”
— The Frozen Ark Project (@frozenark) March 21, 2019 The International Union for the Conservation of Nature’s Red List, in 2017, classified jaguars as “near threatened” — a grade above vulnerable — though their population is on the decline and their habitat “severely fragmented.” Like some of the wild-born cats living at the Brazilian Center for the Conservation of Neotropical Felines in Jundiai, Bianca couldn’t be reintroduced to the wild, Cristina reportedly said.
Following AI with freshly collected semen from a resident wild-born male, Bianca conceived and gave birth to a single viable jaguar cub
Kitty? pic.twitter.com/uYxo6LUFyU — Pixiedust (@PixiedustJtT) October 29, 2021 Lindsey explained that unfrozen jaguar semen only stays good for a few hours. Nevertheless, frozen semen can be used for years, but typically has a lower success rate for felines than with humans. Semen cryopreservation (a process where biological materials are frozen for preservation) offers greater advantages by allowing the transfer of genetic material, rather than living animals, between geographically distant populations and ensures that genetic diversity is not lost if the animals die without reproducing, CREW explained. Similarly, the use of frozen semen for AI provides an alternative to importing founder animals from other countries, CREW added. Despite the initial sad outcome of Bianca’s cub, scientists haven’t been deterred from AI, with even more advanced procedures like in vitro fertilization (IVF) being viewed as a potential way to save threatened species. Share icon Image credits: Paulo B. Chaves In January, scientists and conservationists successfully transferred an embryo into a southern white rhino surrogate. Consequently, the rhino surrogate became the world’s first rhino to become pregnant via IVF, offering hope for the nearly-extinct species. And, in a recent paper published earlier this month, scientists conducted a study to help protect Neotropical primates, many of which are currently threatened with extinction. Researchers focused on developing a method to freeze and preserve the semen of black howler monkeys for future use in breeding programs.
Scientists recently published a paper detailing efforts to freeze and preserve black howler monkey semen for conservation purposes
— CBS News (@CBSNews) May 28, 2024 They collected semen from five adult males and tested two different freezing solutions, one based on egg yolk and the other on soy lecithin (a fatty substance derived from soybeans), each with two different concentrations of glycerol (a sweet, thick liquid used to keep things moist and mix ingredients). The study ultimately found that using egg yolk was better than soy lecithin for keeping the sperm healthy. Also, using a higher amount of glycerol in the egg yolk mix seemed to give the best results. As a result, the research provided new insights into how to preserve monkey sperm.
The research left some readers divided
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